Gender-related barriers in health access and delivery are rooted within political, economic, social, and legal systems These include restrictive gender norms, resource constraints such as for time and finances, and the distribution of power, autonomy, and participation in decision-making, at domestic, community, institutional and structural levels. Comprehensive and cohesive action plans must be implemented that counter the gender-related barriers and inequalities in vaccine delivery and access A guidance note and checklist is provided to undertake this work